The primary sequence of Ricinus communis agglutinin. Comparison with ricin.
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چکیده
منابع مشابه
Characterization of Ricin and R. communis Agglutinin Reference Materials
Ricinus communis intoxications have been known for centuries and were attributed to the toxic protein ricin. Due to its toxicity, availability, ease of preparation, and the lack of medical countermeasures, ricin attracted interest as a potential biological warfare agent. While different technologies for ricin analysis have been established, hardly any universally agreed-upon "gold standards" ar...
متن کاملChemical modification studies on Ricinus communis (Castor Bean) agglutinin.
Ricinus communis agglutinin was subjected to various chemical treatments and the effect on its hemagglutinating and saccharide-binding properties was studied. Acetylation, succinylation and citraconylation led to a complete loss in the activity of the agglutinin, whereas reductive methylation had no effect on the activity, showing that charged amino groups were involved in the hemagglutinating ...
متن کاملCaptured Proteins by Immobilized Ricinus Communis Agglutinin in Rabbit Plasma
The Sepharose CL-6B column was prepared to separate ricinus communis agglutinin (RCA) from the crude extract of castor beans due to the gel carrier contains a large amount of β-galactoside bond. RCA were immobilized on activated Sepharose 4B to make an immobilized RCA column, which was used to capture the proteins in rabbit plasma by affinity chromatography. Results showed that two polypeptides...
متن کاملLipolytic activity of ricin from Ricinus sanguineus and Ricinus communis on neutral lipids.
The present study was carried out with a view of determining ricin lipolytic activity on neutral lipids in emulsion and in a membrane-like model. Using 2,3-dimercapto-1-propanol tributyrate (BAL-TC(4)) as substrate, the lipolytic activity of ricin was found to be proportional to ricin and substrate concentrations, with an apparent K(m) (K(m,app)) of 2.4 mM, a k(cat) of 200 min(-1) and a specifi...
متن کاملEvaluation of the stoichiometry and energetics of carbohydrate binding to Ricinus communis agglutinin: a calorimetric study.
High-sensitivity isothermal titration calorimetry has been used to investigate the thermodynamics of binding of Ricinus communis agglutinin to galactose, lactose and their derivatives in the temperature range 280.5-298 K. The present study unequivocally establishes the carbohydrate-binding stoichiometry of the tetrameric agglutinin from castor bean as two, i.e. the (As-sB)2-type tetramer of the...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1985
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)36312-3